Catalase Reagent

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Catalase Reagent is useful in the presumptive identification and differentiation of many bacteria.
Description

INTENDED USE

Hardy Diagnostics Catalase Reagent is useful in the presumptive identification and differentiation of many bacteria. Beta-hemolytic organisms, such as Streptococcus species (catalase-negative), Staphylococcus species (catalase-positive), and Listeria species (catalase-positive) can be differentiated by their catalase reaction.

SUMMARY

Most cytochrome containing organisms produce a catalase enzyme which breaks down hydrogen peroxide into oxygen and water. When a small amount of a catalase producing organism is introduced into hydrogen peroxide, bubbles of oxygen form as a result of the enzyme's activity.

REAGENT FORMULA

Hydrogen Peroxide, 3%, and Acetophenetidine.

PROCEDURE

Specimen Collection: This product is not intended for primary isolation of patient specimens. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.Method of Use: With a loop or sterile wooden stick, transfer a small amount of a well isolated, 18 to 24 hour old colony from a non-blood-containing agar onto the surface of a clean, dry glass slide. Immediately place a drop of Catalase Reagent onto a portion of the colony on the slide.

INTERPRETATION OF RESULTS

Observe for the evolution of gas bubbles indicating a positive test. 

Catalase Test

Hydrogen peroxide 3%, detects catalase activity, with dropper cap. 

 

Cat. no. HDZ62 / 15mL

Cat. no. HDZ76 / 60mL

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