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Enumeration for Vibrio parahaemolyticus
Description

A SIMPLE TO USE COUNTING METHOD FOR VIBRIO


Compact Dry VP is an easy to use counting system for Vibrio parahaemolyticus in food and environmental samples. Compact Dry VP can not only easily detect Vibrio parahaemolyticus, but also differentiate Vibrio parahaemolyticus from other vibrios.

Vibrio parahaemolyticus is a bacterium that can cause cholera. Vibrio parahaemolyticus-associated gastroenteritis is the infection caused by this organism. Vibrio parahaemolyticus naturally inhabits coastal waters and is present in higher concentrations during the summer; it is a halophilic, or salt-requiring organism. Vibrio parahaemolyticus is found in marine environments, seafoods, and the feces of patients with acute enteritis. 

Results are available in 20-24 hours and are incubated at 35-37°C.   

Easy-to-read-results test

The media contains a specific chromogenic substrate for Vibrio parahaemolyticus which develops blue/green or blue colonies, whereas other vibrios develop white colonies.

Easy-to-store test

The plates can be kept at room temperature for up to 15 months after production.

Each foil bag contains 4 tests, ideal for small or infrequent users.

Advantages

  • Room temperature storage
  • Sample is self diffusible, not need for the spreader plate
  • Easier and faster to use than any other method.
  • No height limits when stacking for  incubation.
  • Easy to subculture suspect colonies
  • Long shelf life
  • No special diluents needed
  • Allows culturing of filter membranes Each foil bag contains 4 tests, ideal for small or infrequent users.

Direction for use 

  1. Open aluminum foil and take out the set of 4 plates.
  2. Detach necessary number of plates by bending up and down while pressing the lid. 
  3. Take off the lid of the plate and pipette 1 ml of sample in the middle of the Compact Dry plate.
  4. Sample diffuses automatically and evenly across the sheet, transforming the dried sheet into a gel within seconds. 
  5. Replace the lid back on the plate and label. Invert the plate and incubate. 
  6. Count the number of colonies appearing on the medium from underside of the plate. When the number of colonies is high, it is convenient to use the grids carved on the back of the container consisting of 1 cm x 1 cm or 0.5 cm x 0.5 cm. 

 

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