Hardy Diagnostics AlbiQuick™ Test Kit is a rapid test for the identification of Candida albicans by detecting the enzymes beta-galactosaminidase and L-proline aminopeptidase in yeasts.
Hardy Diagnostics AlbiQuick™ Test Kit is based on the ability of yeast species to rapidly hydrolyze the compounds proline-p-nitroanilide (PRO) using the enzyme L-proline aminopeptidase; and 4-methylumbelliferyl-n-acetyl-beta-galactosamine (NAG), using the enzyme beta-galactosaminidase. The test card consists of two separate test circles each containing one of the substrates. When hydrolyzed, the PRO substrate forms a colored end product, and if the NAG substrate is hydrolyzed it produces a fluorescent reaction. Candida albicans produces both enzymes, while other commonly encountered yeasts may be positive for one or the other enzyme but not for both. The total incubation time for AlbiQuick™ is only six minutes.
Circle A is impregnated with 4-methylumbelliferyl-n-acetyl-beta-galactosamine dissolved in a solvent. Circle B is impregnated with proline-p-nitroanilide dissolved in a solvent. The developers, Reagent A and Reagent B, consist of p-dimethylaminocinnamaldehyde and phosphate buffer, respectively.
Use a culture that is between 24 to 96 hours old to inoculate the AlbiQuick™ Test Card. Isolates may be tested from primary isolation media such as Blood Agar (Cat. no. A10), Chocolate Agar (Cat. no. E14) or may be subcultured onto Sabouraud Dextrose Agar (Cat. no. W70) incubated at room temperature before being tested.
1. Moisten both Circle A and Circle B with one drop of deionized water. Do not oversaturate the test area.
2. Using a sterile loop or applicator stick, remove a heavy, visible paste of yeast cells (2-3 yeast colonies), and smear the paste onto Circle A. Repeat this step for Circle B.
3. Incubate the test card for 5 minutes at room temperature (15-30 degrees C.).
4. After incubation, add one drop of Reagent B to Circle B. The development of a blue-purple color within 30 seconds (usually immediately) is a positive test for PRO hydrolysis. If no color develops, the test is negative.
5. Add one drop of Reagent A to Circle A. After 30 seconds, examine Circle A under long-wave UV light (366nm). A bright, blue-white fluorescence is a positive test for the hydrolysis of NAG. If no fluorescence is present, the test is negative.
INTERPRETATION OF RESULTS
A positive test for NAG hydrolysis (Circle A) is the appearance of a blue-white fluorescence. No fluorescence is a negative NAG test.A positive test for PRO hydrolysis (Circle B) is the appearance of a blue-purple color. No change in color is a negative PRO test.A positive reaction in both circles is a presumptive identification of Candida albicans. Other yeast species will yield negative reactions in one or both circles.
Cat No. HDZ121
Size: 25 Tests