CarboFerm Neisseria Kit

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Rapid test ID for Neisseria species
Description

INTENDED USE

Hardy Diagnostics CarboFerm™ Neisseria Kit is a rapid test (four hour) for the identification of Neisseria species (including N. gonorrhoeae and N. meningitidis) and Moraxella (Branhamella) catarrhalis. CarboFerm™ uses acid production from carbohydrates and the presence of butyrate esterase to differentiate and identify Neisseria species and M. catarrhalis.

SUMMARY

The majority of Neisseria species are considered normal flora of mucous membranes on humans. Neisseria gonorrhoeae is a sexually transmitted pathogen and Neisseria meningitidis is often associated with meningitis as well as colonization of the nasopharynx. M. catarrhalis causes respiratory tract and other infections.Clinical Laboratory Standards Institute (CLSI - formerly NCCLS) recommends using the butyrate esterase test to identify M. catarrhalis.(9) 

PROCEDURE

Specimen Collection: This product is not intended for primary isolation of patient specimens.The appropriate organisms for performing the CarboFerm™ Neisseria test are oxidase-positive, gram-negative diplococci. 

Method of use:

1. Remove caps from the test strips to be used. Leave strips in the base. Unused strips may be removed from the base and stored in the bag (with caps on) until needed.

2. Use a sterile swab or loop to transfer a pure isolate (from Chocolate, Thayer Martin, or Martin Lewis agar) into a tube of Inoculation Buffer. The culture should be no older than 24 hours. Prepare a heavy suspension (equivalent to a 4.0 McFarland turbidity standard or higher). Vortex and/or aspirate repeatedly until density of the solution is uniform in appearance. A low density may result in false-negative reactions.

3. Aseptically transfer 4 to 5 drops (or approximately 0.18ml) of the suspension into each well of the strip.

Do not add any suspension to the second (B) or seventh (G) wells. These wells are intentionally left empty. Do not remove the test strips from the base holder.4. Incubate strips uncovered at 35 degrees C. aerobically. Do not incubate in a CO2 atmosphere. Read the butyrate well (row H) after 10 to 15 minutes. Read the carbohydrate wells (row A, C, D, E, and F) after four hours and before seven hours (some positives will appear in as little as two hours).

INTERPRETATION OF RESULTS

The reaction in row H (butyrate) should be read after 10 to 15 minutes at 35 degrees C. A blue coloration is considered positive.


Note: Color changes that take place in the butyrate well (H) after 15 minutes of incubation time should be disregarded.Positive reactions can be read in as little as two hours. Negative carbohydrate wells should be held for a minimum of four hours. A change of color from red to orange or yellow is considered positive for the carbohydrate wells (wells C through F). Well A is used as a reference and should remain negative (red) throughout incubation, since it contains no carbohydrate. Read final results at four hours.
Note: Color changes in the carbohydrate wells that take place after seven hours of incubation time should be disregarded.
A carbohydrate reaction which is more yellow or orange than the control well (A) is considered positive.Use the chart below to identify the listed organisms.

 

Organism

Negative
Control
(well A)

Glucose
(well C)

Maltose
(well D)

Lactose
(well E)

Sucrose
(well F)

Butyrate
(well H)

N. gonorrhoeae / cinerea*

-
red

+
orange or
yellow

-
red

-
red

-
red

-
white

N. meningitidis

-
red

+
orange or
yellow

+
orange or
yellow

-
red

-
red

-
white

N. lactamica

-
red

+
orange or
yellow

+
orange or
yellow

+
orange or
yellow

-
red

-
white

N. sicca / N. mucosa**

-
red

+
orange or
yellow

+
orange or
yellow

-
red

+
orange or
yellow

-
white

M. catarrhalis

-
red

-
red

-
red

-
red

-
red

+
blue

 

* N. cinerea may produce a false-positive glucose. This has not been observed with CarboFerm™, however, N. cinerea will not usually grow on Thayer Martin Agar but will grow on Nutrient Agar. N. gonorrhoeae will grow on Thayer Martin Agar but will not grow on Nutrient Agar.** To distinguish between N. sicca and N. mucosa, perform a nitrate reduction test (Cat. no. K42). N. mucosa is positive for nitrate reduction and N. sicca is negative.  

Cat No. HDZ98

Size: 24 Tests

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